Application of ultraviolet germicidal lamp in automatic nucleic acid extraction instrument
#We are acting against incidents# According to statistics released by the national and local health commissions daily, as of 2020.4.5, a total of 82,930 cases of new coronary pneumonia have been confirmed in the country, a total of 77,237 cases have been cured, and 2355 cases have been confirmed so far. In 316 cities, the existing confirmed cases have been cleared. The Joint Prevention and Control Office of the National Health Commission stated: The peak of the current epidemic in my country has passed, the number of new cases has continued to decline, the overall epidemic situation has remained at a low level, and foreign prevention has been imported. It has become the top priority of my country's epidemic prevention and control. In the face of the new crown pneumonia epidemic, under the current medical technology system in my country and around the world, in the extraction of virus samples, nucleic acid extraction testing and CT testing have become important detection methods for COVID-19 coronavirus. The automatic nucleic acid extractor is an instrument that uses matching nucleic acid extraction reagents to automatically complete the sample nucleic acid extraction work. It is an indispensable instrument for the separation and purification of biomolecules in each laboratory. Nucleic acid extractors are divided into two categories. One type is a large-scale automated laboratory experiment instrument, generally called an automatic liquid workstation; the other type is a small-scale automatic nucleic acid extraction instrument, which uses packaged supporting reagents to automatically complete the extraction and purification process.
Take the working principle of the automatic nucleic acid extractor as an example. When the automatic nucleic acid extraction system is working, aerosol pollution will occur. The World Health Organization report once pointed out that viruses or bacteria can spread through aerosols over long distances and cause large-scale infections in a short period of time. Looking back on the past, industry insiders pointed out that researchers have found clear evidence of SARS virus transmission through air aerosols. Secondly, the H5N1 avian influenza virus also has evidence of transmission through aerosol routes. In view of the 80% genetic sequence similarity between the new coronavirus and SARS, it is speculated that the physical properties of the new coronavirus and SARS may be similar, and the possibility of aerosol transmission is not ruled out. In the detection of COVID-19 coronavirus nucleic acid, DNA pollution existing in laboratory desktops, instruments, consumables and air will cause serious interference to the detection experiment, especially the DNA molecules carried by aerosols (an aerosol can carry Thousands of DNA), floating in the air, difficult to eliminate. During the PCR amplification process, the problem of DNA/RNA aerosol contamination will occur. A very small amount of aerosol pollution can cause false positive results, which may cause contamination of the entire PCR laboratory, resulting in a high probability of false negative events in the detection of new coronary pneumonia nucleic acid, and even close the laboratory in serious cases.
